Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays

Giraud, Gerard, Schulze, Holger, Bachmann, Till T., Campbell, Colin J., Mount, Andrew R., Ghazal, Peter, Khondoker, Mizanur R., Ross, Alan J., Ember, Stuart W. J., Ciani, Ilenia, Tlili, Chaker, Walton, Anthony J., Terry, Jonathan G. and Crain, Jason (2009) Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays. International Journal of Molecular Sciences, 10 (4). pp. 1930-1941. ISSN 1661-6596

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Abstract

Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.

Item Type: Article
Uncontrolled Keywords: quantum dots (qd),fluorescence lifetime imaging microscopy (flim),dna microarray,lifetime multiplexing
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 24 Sep 2016 00:41
Last Modified: 26 Jul 2020 23:40
URI: https://ueaeprints.uea.ac.uk/id/eprint/60179
DOI: 10.3390/ijms10041930

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