Siglec-F is a novel intestinal M cell marker

Gicheva, Nadezhda, Macauley, Matthew S., Arlian, Britni M., Paulson, James C. and Kawasaki, Norihito (2016) Siglec-F is a novel intestinal M cell marker. Biochemical and Biophysical Research Communications, 479. 1–4. ISSN 0006-291X

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Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M-cells, demonstrating the potential to target M cells via Siglec-F.

Item Type: Article
Uncontrolled Keywords: siglec,m cell,oral vaccine,peyer's patch
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 24 Sep 2016 00:22
Last Modified: 24 Jul 2019 22:40
URI: https://ueaeprints.uea.ac.uk/id/eprint/60015
DOI: 10.1016/j.bbrc.2016.08.055

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