Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy

Bai, Jie, Wang, Julie Tzu-Wen, Mei, Kuo-Ching, Al-Jamal, Wafa T. and Al-jamal, Khuloud T. (2016) Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy. Journal of Controlled Release, 244 (Part B). 240–246. ISSN 0168-3659

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Abstract

Magnetic drug targeting has been proposed as means of concentrating therapeutic agents at a target site and the success of this approach has been demonstrated in a number of studies. However, the behavior of magnetic carriers in blood vessels and tumor microcirculation still remains unclear. In this work, we utilized polymeric magnetic nanocapsules (m-NCs) for magnetic targeting in tumors and dynamically visualized them within blood vessels and tumor tissues before, during and after magnetic field exposure using fibered confocal fluorescence microscopy (FCFM). Our results suggested that the distribution of m-NCs within tumor vasculature changed dramatically, but in a reversible way, upon application and removal of a magnetic field. The m-NCs were concentrated and stayed as clusters near a blood vessel wall when tumors were exposed to a magnetic field but without rupturing the blood vessel. The obtained FCFM images provided in vivo in situ microvascular observations of m-NCs upon magnetic targeting with high spatial resolution but minimally invasive surgical procedures. This proof-of-concept descriptive study in mice is envisaged to track and quantify nanoparticles in vivo in a non-invasive manner at microscopic resolution.

Item Type: Article
Additional Information: Published in the 14th edition of the European Symposium on Controlled Drug Delivery, Egmond aan Zee, The Netherlands on April 13-15, 2016 This article is available under the terms of the Creative Commons Attribution License (CC BY). You may copy and distribute the article, create extracts, abstracts and new works from the article, alter and revise the article, text or data mine the article and otherwise reuse the article commercially (including reuse and/or resale of the article) without permission from Elsevier. You must give appropriate credit to the original work, together with a link to the formal publication through the relevant DOI and a link to the Creative Commons user license above. You must indicate if any changes are made but not in any way that suggests the licensor endorses you or your use of the work.
Uncontrolled Keywords: superparamagnetic iron oxide nanoparticles (spions),magnetic drug targeting,cellvizio®,tumor,nanomedicine
Faculty \ School: Faculty of Science > School of Pharmacy
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Depositing User: Pure Connector
Date Deposited: 24 Sep 2016 00:12
Last Modified: 24 Jul 2019 22:36
URI: https://ueaeprints.uea.ac.uk/id/eprint/59905
DOI: 10.1016/j.jconrel.2016.07.026

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