c-Fos induction by gut hormones and extracellular ATP in osteoblastic-like cell lines

Pacheco-Pantoja, Elda Leonor, Dillon, Jane P., Wilson, Peter J. M., Fraser, William D. and Gallagher, James A. (2016) c-Fos induction by gut hormones and extracellular ATP in osteoblastic-like cell lines. Purinergic Signalling, 12 (4). 647–651. ISSN 1573-9538

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It is widely accepted that the c-Fos gene has a role in proliferation and differentiation of bone cells. ATP-induced c-Fos activation is relevant to bone homeostasis, because nucleotides that are present in the environment of bone cells can contribute to autocrine/paracrine signalling. Gut hormones have previously been shown to have an effect on bone metabolism. In this study, we used the osteoblastic Saos-2 cell line transfected with a c-Fos-driven reporter stimulated with five gut hormones: glucose inhibitory peptide (GIP), glucagon-like peptide-1 (GLP-1), glucagon-like peptide-2 (GLP-2), ghrelin and obestatin, in the presence or absence of ATP. In addition, TE-85 cells were used to determine the time course of c-Fos transcript induction following stimulation with GLP-1, and GLP-2 with or without ATP, using reverse transcription qPCR. The significant results from the experiments are as follows: higher level of c-Fos induction in presence of GIP, obestatin (p = 0.019 and p = 0.011 respectively), and GIP combined with ATP (p < 0.001) using the luciferase assay; GLP-1 and GLP-2 combined with ATP (p = 0.034 and p = 0.002, respectively) and GLP-2 alone (p < 0.001) using qPCR. In conclusion, three of the gut peptides induced c-Fos, providing a potential mechanism underlying the actions of these hormones in bone which can be directed or enhanced by the presence of ATP.

Item Type: Article
Uncontrolled Keywords: atp,gip,glp-1,glp-2,osteoblasts,c-fos
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User: Pure Connector
Date Deposited: 24 Sep 2016 00:08
Last Modified: 19 Oct 2023 01:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/59854
DOI: 10.1007/s11302-016-9526-3


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