A novel broadly applicable PCR-RFLP method for rapid identification and subtyping of H58 Salmonella Typhi

Murgia, Manuela, Rubino, Salvatore, Wain, John, Gaind, Rajni and Paglietti, Bianca (2016) A novel broadly applicable PCR-RFLP method for rapid identification and subtyping of H58 Salmonella Typhi. Journal of Microbiological Methods, 127. pp. 219-223. ISSN 0167-7012

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Abstract

Salmonella Typhi (S. Typhi), the human-adapted agent of typhoid fever, is genetically monomorphic. SNPs accumulation divided the S. Typhi population in 85 haplotypes (H) of which one, H58, has undergone a clonal expansion. The surveillance of H58 S. Typhi is particularly important, especially in areas where typhoid fever is endemic. We developed a simple PCR and PCR-RFLP method to detect and subtype H58 S. Typhi based on the presence of genomic deletion and specific SNPs. The method was validated against 39 S. Typhi isolates of known haplotype, showing 100% of specificity and high sensitivity, and then used to screen a collection of 99 S. Typhi from Asia, demonstrating a high incidence of H58 S. Typhi in Jordan and India. Our method is designed to be applied in all laboratories with basic molecular biology equipment and few financial resources and allows the surveillance of H58 S. Typhi in resource poor settings.

Item Type: Article
Uncontrolled Keywords: s. typhi,snps,phylogenetic tree,h58 identification,pcr-rflp
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: Pure Connector
Date Deposited: 24 Sep 2016 00:04
Last Modified: 24 Nov 2020 00:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/59796
DOI: 10.1016/j.mimet.2016.06.018

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