Characterization of Ferroplasma Isolates and Ferroplasma acidarmanus sp. nov., Extreme Acidophiles from Acid Mine Drainage and Industrial Bioleaching Environments

Dopson, Mark, Baker-Austin, Craig, Hind, Andrew, Bowman, John P. and Bond, Philip L. (2004) Characterization of Ferroplasma Isolates and Ferroplasma acidarmanus sp. nov., Extreme Acidophiles from Acid Mine Drainage and Industrial Bioleaching Environments. Applied and Environmental Microbiology, 70 (4). pp. 2079-2088.

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Abstract

Three recently isolated extremely acidophilic archaeal strains have been shown to be phylogenetically similar to Ferroplasma acidiphilum YT by 16S rRNA gene sequencing. All four Ferroplasma isolates were capable of growing chemoorganotrophically on yeast extract or a range of sugars and chemomixotrophically on ferrous iron and yeast extract or sugars, and isolate "Ferroplasma acidarmanus" Fer1T required much higher levels of organic carbon. All four isolates were facultative anaerobes, coupling chemoorganotrophic growth on yeast extract to the reduction of ferric iron. The temperature optima for the four isolates were between 35 and 42{degrees}C and the pH optima were 1.0 to 1.7, and "F. acidarmanus" Fer1T was capable of growing at pH 0. The optimum yeast extract concentration for "F. acidarmanus" Fer1T was higher than that for the other three isolates. Phenotypic results suggested that isolate "F. acidarmanus" Fer1T is of a different species than the other three strains, and 16S rRNA sequence data, DNA-DNA similarity values, and two-dimensional polyacrylamide gel electrophoresis protein profiles clearly showed that strains DR1, MT17, and YT group as a single species. "F. acidarmanus" Fer1T groups separately, and we propose the new species "F. acidarmanus" Fer1T sp. nov.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science
Faculty of Science > School of Environmental Sciences
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Depositing User: Pure Connector
Date Deposited: 24 Jun 2016 16:00
Last Modified: 24 Jul 2019 16:39
URI: https://ueaeprints.uea.ac.uk/id/eprint/59600
DOI: 10.1128/AEM.70.4.2079-2088.2004

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