The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification

Hatch, Victoria, Marin Barba, Marta, Moxon, Simon, Ford, Christopher, Ward, Nicole, Tomlinson, Matthew, Desanlis, Ines, Hendry, Adam, Hontelez, Saartje, van Krujsbergen, Ila, Veenstra, Gert Jan, Munsterberg, Andrea and Wheeler, Grant (2016) The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification. Developmental Biology, 416 (2). 361–372. ISSN 0012-1606

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Abstract

Regulation of gene expression at the level of transcriptional elongation has been shown to be important in stem cells and tumour cells, but its role in the whole animal is only now being fully explored. Neural crest cells (NCCs) are a multipotent population of cells that migrate during early development from the dorsal neural tube throughout the embryo where they differentiate into a variety of cell types including pigment cells, cranio-facial skeleton and sensory neurons. Specification of NCCs is both spatially and temporally regulated during embryonic development. Here we show that components of the transcriptional elongation regulatory machinery, CDK9 and CYCLINT1 of the P-TEFb complex, are required to regulate neural crest specification. In particular, we show that expression of the proto-oncogene c-Myc and c-Myc responsive genes are affected. Our data suggest that P-TEFb is crucial to drive expression of c-Myc, which acts as a ‘gate-keeper’ for the correct temporal and spatial development of the neural crest.

Item Type: Article
Uncontrolled Keywords: neural crest cells,leflunomide,transcriptional elongation,c-myc,p-tefb,xenopus,polymerase pausing,cdk9,cyclint1
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Computing Sciences
Faculty of Science
Depositing User: Pure Connector
Date Deposited: 15 Jun 2016 14:00
Last Modified: 25 Jun 2020 00:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/59361
DOI: 10.1016/j.ydbio.2016.06.012

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