Editing plant genomes with CRISPR/Cas9

Belhaj, Khaoula, Chaparro-garcia, Angela, Kamoun, Sophien, Patron, Nicola J and Nekrasov, Vladimir (2015) Editing plant genomes with CRISPR/Cas9. Current Opinion in Biotechnology, 32. pp. 76-84. ISSN 0958-1669

Full text not available from this repository. (Request a copy)

Abstract

CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.

Item Type: Article
Faculty \ School:
Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 06 Jun 2016 16:00
Last Modified: 22 Apr 2020 01:28
URI: https://ueaeprints.uea.ac.uk/id/eprint/59231
DOI: 10.1016/j.copbio.2014.11.007

Actions (login required)

View Item View Item