Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing

Witek, Kamil, Jupe, Florian, Witek, Agnieszka, Baker, David, Clark, Matthew D. and Jones, Jonathan D.G. (2016) Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing. Nature Biotechnology, 34 (6). 656–660. ISSN 1087-0156

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Abstract

Global yields of potato and tomato crops are reduced owing to potato late blight disease, which is caused by Phytophthora infestans. Although most commercial potato varieties are susceptible to blight, wild potato relatives are not and are therefore a potential source of Resistance to P. infestans (Rpi) genes. Resistance breeding has exploited Rpi genes from closely related tuber-bearing potato relatives, but is laborious and slow 1–3. Here we report that the wild, diploid non-tuber-bearing Solanum americanum harbors multiple Rpi genes. We combine R gene sequence capture (RenSeq4) with single-molecule real-time SMRT sequencing (SMRT RenSeq) to clone Rpi-amr3i . This technology should enable de novo assembly of complete nucleotide-binding, leucine-rich repeat receptor (NLR) genes, their regulatory elements and complex multi-NLR loci from uncharacterized germplasm. SMRT RenSEQ can be applied to rapidly clone multiple R genes for engineering pathogen-resistant crops.

Item Type: Article
Faculty \ School:
Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 27 Apr 2016 15:00
Last Modified: 25 Nov 2020 00:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/58440
DOI: 10.1038/nbt.3540

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