High-throughput-sequencing-based identification of a grapevine fanleaf virus satellite RNA in Vitis vinifera

Chiumenti, Michela, Mohorianu, Irina, Roseti, Vincenzo, Saldarelli, Pasquale, Dalmay, Tamas and Minafra, Angelantonio (2016) High-throughput-sequencing-based identification of a grapevine fanleaf virus satellite RNA in Vitis vinifera. Archives of Virology, 161 (5). pp. 1401-1403. ISSN 0304-8608

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Abstract

A new satellite RNA (satRNA) of grapevine fanleaf virus (GFLV) was identified by high-throughput sequencing of high-definition (HD) adapter libraries from grapevine plants of the cultivar Panse precoce (PPE) affected by enation disease. The complete nucleotide sequence was obtained by automatic sequencing using primers designed based on next-generation sequencing (NGS) data. The full-length sequence, named satGFLV-PPE, consisted of 1119 nucleotides with a single open reading frame from position 15 to 1034. This satRNA showed maximum nucleotide sequence identity of 87 % to satArMV-86 and satGFLV-R6. Symptomatic grapevines were surveyed for the presence of the satRNA, and no correlation was found between detection of the satRNA and enation symptom expression.

Item Type: Article
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 22 Apr 2016 10:00
Last Modified: 22 Apr 2020 01:15
URI: https://ueaeprints.uea.ac.uk/id/eprint/58337
DOI: 10.1007/s00705-016-2776-0

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