Maternal exposure to a Western-style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups

Steegenga, Wilma T., Mischke, Mona, Lute, Carolien, Boekschoten, Mark V., Lendvai, Agnes, Pruis, Maurien G. M., Verkade, Henkjan J., van de Heijning, Bert J. M., Boekhorst, Jos, Timmerman, Harro M., Plösch, Torsten, Muller, Michael ORCID: https://orcid.org/0000-0002-5930-9905 and Hooiveld, Guido JEJ (2017) Maternal exposure to a Western-style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups. Molecular Nutrition & Food Research, 61 (1). ISSN 1613-4125

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Abstract

Scope:The long-lasting consequences of nutritional programming during the early phase of life have become increasingly evident. The effects of maternal nutrition on the developing intestine are still underexplored. Methods and results: In this study we observed 1) altered microbiota composition of the colonic luminal content, and 2) differential gene expression in the intestinal wall in two-week-old mouse pups born from dams exposed to a Western-style (WS) diet during the perinatal period. A sexually dimorphic effect was found for the differentially expressed genes in the offspring of WS diet-exposed dams but no differences between male and female pups were found for the microbiota composition. Integrative analysis of the microbiota and gene expression data revealed that the maternal WS diet independently affected gene expression and microbiota composition. However, the abundance of bacterial families not affected by the WS diet (Bacteroidaceae, Porphyromonadaceae and Lachnospiraceae) correlated with the expression of genes playing a key role in intestinal development and functioning (e.g. Pitx2 and Ace2). Conclusion: Our data reveal that maternal consumption of a WS diet during the perinatal period alters both gene expression and microbiota composition in the intestinal tract of two-week-old offspring.

Item Type:	Article
Additional Information:	Published in Special Issue: Gut Microbiota, Diet and Health © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords:	gut development,maternal diet,microbiota composition,offspring,transcriptomics
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	14 Apr 2016 16:00
Last Modified:	22 Oct 2022 01:01
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58251
DOI:	10.1002/mnfr.201600141

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