A role for the pattern recognition receptor Nod2 in promoting recruitment of CD103+ dendritic cells in the colon in response to Trichuis muris infection

Bowcutt, L, Bramhall, L, Longunova, L, Booth, C, Carding, SR, Grencis, R and Cruickshank, S (2014) A role for the pattern recognition receptor Nod2 in promoting recruitment of CD103+ dendritic cells in the colon in response to Trichuis muris infection. Mucosal Immunology, 7. pp. 1094-1105. ISSN 1933-0219

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Abstract

The ability of the colon to generate an immune response to pathogens, such as the model pathogen Trichuris muris, is a fundamental and critical defense mechanism. Resistance to T. muris infection is associated with the rapid recruitment of dendritic cells (DCs) to the colonic epithelium via epithelial chemokine production. However, the epithelial–pathogen interactions that drive chemokine production are not known. We addressed the role of the cytosolic pattern recognition receptor Nod2. In response to infection, there was a rapid influx of CD103+CD11c+ DCs into the colonic epithelium in wild-type(WT)mice, where as this was absent in Nod2−/− animals. In vitro chemotaxis assays and in vivo experiments using bone marrow chimeras of WT mice reconstituted with Nod2−/− bone marrow and infected with T. muris demonstrated that the migratory function of Nod2−/− DCs was normal. Investigation of colonic epithelial cell (CEC) innate responses revealed a significant reduction in epithelial production of the chemokines CCL2 and CCL5 but not CCL20 by Nod2-deficient CECs. Collectively, these data demonstrate the importance of Nod2 in CEC responses to infection and the requirement for functional Nod2 in initiating host epithelial chemokine-mediated responses and subsequent DC recruitment and T-cell responses following infection.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 14 Apr 2016 13:00
Last Modified: 28 Oct 2019 11:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/58246
DOI: 10.1038/mi.2013.125

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