Synthesis of diagnostic silicon nanoparticles for targeted delivery of thiourea to epidermal growth factor receptor-expressing cancer cells

Behray, Mehrnaz, Webster, Carl A, Pereira, Sara, Ghosh, Paheli, Krishnamurthy, Satheesh, Al-Jamal, Wafa T and Chao, Yimin (2016) Synthesis of diagnostic silicon nanoparticles for targeted delivery of thiourea to epidermal growth factor receptor-expressing cancer cells. ACS Applied Materials & Interfaces, 8 (14). 8908–8917. ISSN 1944-8244

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Abstract

The novel thiourea-functionalized silicon nanoparticles (SiNPs) have been successfully synthesized using allylamine and sulforaphane, an important anticancer drug, followed by a hydrosilylation reaction on the surface of hydrogen terminated SiNPs. Their physiochemical properties have been investigated by photoluminescence emission, FTIR and elemental analysis. MTT assay has been employed to evaluate in vitro toxicity in colorectal cancer cells (Caco-2) and primary normal cells (CCD). The results show significant toxicity of thiourea SiNPs after 72 h incubation in the cancer cell line and the toxicity is concentration dependent and saturated for concentrations above 100 µg/mL. Confocal microscopy images have demon-strated the internalization of thiourea-functionalized SiNPs inside the cells. Flow cytometry data has confirmed receptor-mediated targeting in cancer cells. This nanocomposite takes advantage of the EGFR active targeting of the ligand in addi-tion to the photoluminescence properties of SiNPs for bioimaging purposes. The results suggest that this novel nanosystem can be extrapolated for active targeting of the receptors that are overexpressed in cancer cells such as EGFR using the targeting characteristics of thiourea-functionalized SiNPs and therefore encourage further investigation and development of anticancer agents specifically exploiting the EGFR inhibitory activity of such nanoparticles.

Item Type: Article
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Chemistry
Faculty of Science
Depositing User: Pure Connector
Date Deposited: 01 Apr 2016 10:14
Last Modified: 28 Oct 2019 15:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/58044
DOI: 10.1021/acsami.5b12283

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