Broad application of a simple and affordable protocol for isolating plant RNA

Couto, Daniel, Stransfeld, Lena, Arruabarrena, Ana, Zipfel, Cyril and Lozano-Durán, Rosa (2015) Broad application of a simple and affordable protocol for isolating plant RNA. BMC Research Notes, 8. ISSN 1756-0500

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Abstract

BACKGROUND: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. FINDINGS: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. CONCLUSIONS: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.

Item Type: Article
Uncontrolled Keywords: arabidopsis,green chemistry technology,guanidines,indicators and reagents,lycopersicon esculentum,phenols,plant leaves,plant rna,triticum
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 09 Mar 2016 16:01
Last Modified: 12 Nov 2019 16:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/57400
DOI: 10.1186/s13104-015-1119-7

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