Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade

Lidbury, Ian, Kimberley, George, Scanlan, David J., Murrell, J. Colin and Chen, Yin (2015) Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade. Environmental Microbiology, 17 (12). pp. 5048-5062. ISSN 1462-2912

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Abstract

Choline is ubiquitous in marine eukaryotes and appears to be widely distributed in surface marine waters; however, its metabolism by marine bacteria is poorly understood. Here, using comparative genomics and molecular genetic approaches, we reveal that the capacity for choline catabolism is widespread in marine heterotrophs of the marine Roseobacter clade (MRC). Using the model bacterium Ruegeria pomeroyi, we confirm that the betA, betB and betC genes, encoding choline dehydrogenase, betaine aldehyde dehydrogenase and choline sulfatase, respectively, are involved in choline metabolism. The betT gene, encoding an organic solute transporter, was essential for the rapid uptake of choline but not glycine betaine (GBT). Growth of choline and GBT as a sole carbon source resulted in the re-mineralization of these nitrogen-rich compounds into ammonium. Oxidation of the methyl groups from choline requires formyltetrahydrofolate synthetase encoded by fhs in R.pomeroyi, deletion of which resulted in incomplete degradation of GBT. We demonstrate that this was due to an imbalance in the supply of reducing equivalents required for choline catabolism, which can be alleviated by the addition of formate. Together, our results demonstrate that choline metabolism is ubiquitous in the MRC and reveal the role of Fhs in methyl group oxidation in R.pomeroyi.

Item Type: Article
Additional Information: © 2015 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution andreproduction in any medium, provided the original work is properly cited.
Faculty \ School:
Faculty of Science > School of Environmental Sciences
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Depositing User: Pure Connector
Date Deposited: 16 Feb 2016 10:00
Last Modified: 22 Jul 2020 00:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/57093
DOI: 10.1111/1462-2920.12943

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