Oakleaf: an S locus-linked mutation of Primula vulgaris that affects leaf and flower development

Cocker, Jonathan, Webster, Margaret, Li, Jinhong, Wright, Jonathan, Kaithakottil, Gemy, Swarbreck, David and Gilmartin, Philip (2015) Oakleaf: an S locus-linked mutation of Primula vulgaris that affects leaf and flower development. New Phytologist, 208 (1). pp. 149-161. ISSN 0028-646X

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Abstract

•In Primula vulgaris outcrossing is promoted through reciprocal herkogamy with insect-mediated cross-pollination between pin and thrum form flowers. Development of heteromorphic flowers is coordinated by genes at the S locus. To underpin construction of a genetic map facilitating isolation of these S locus genes, we have characterised Oakleaf, a novel S locus-linked mutant phenotype. •We combine phenotypic observation of flower and leaf development, with classical genetic analysis and next-generation sequencing to address the molecular basis of Oakleaf. •Oakleaf is a dominant mutation that affects both leaf and flower development; plants produce distinctive lobed leaves, with occasional ectopic meristems on the veins. This phenotype is reminiscent of overexpression of Class I KNOX-homeodomain transcription factors. We describe the structure and expression of all eight P. vulgaris PvKNOX genes in both wild-type and Oakleaf plants, and present comparative transcriptome analysis of leaves and flowers from Oakleaf and wild-type plants. •Oakleaf provides a new phenotypic marker for genetic analysis of the Primula S locus. We show that none of the Class I PvKNOX genes are strongly upregulated in Oakleaf leaves and flowers, and identify cohorts of 507 upregulated and 314 downregulated genes in the Oakleaf mutant.

Item Type: Article
Uncontrolled Keywords: knox genes,heterostyly,oakleaf,primula vulgaris,s locus
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science
Depositing User: Pure Connector
Date Deposited: 26 Jan 2016 12:00
Last Modified: 28 Jul 2019 00:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/56767
DOI: 10.1111/nph.13370

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