Two-photon fluorescence: Resonance energy transfer

Allcock, Philip and Andrews, David L. (1998) Two-photon fluorescence: Resonance energy transfer. The Journal of Chemical Physics, 108 (8). pp. 3089-3095. ISSN 0021-9606

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Fluorescence resonance energy transfer (FRET) is a technique now widely applied to probe biological and other complex systems for the determination of fluorophore separation and structure. Recently the theory behind the anisotropy of fluorescence has been extended to include the case of a residual polarization following energy transfer between fluorophores, and here the theory is further extended to accommodate two-photon excitation. This reveals not only novel polarization characteristics but also a distance dependence whose analysis does not require a priori knowledge of the donor–acceptor spectral overlap. The two-photon FRET anisotropy results mirror their one-photon counterparts, in terms of fluorophore separation characteristics and also their relationship to the anisotropies for isolated fluorophores. Moreover, the two-photon results are not restricted to a plane polarized input, results being given for both plane and circularly polarized pump radiation.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Depositing User: Pure Connector
Date Deposited: 18 Jan 2016 17:03
Last Modified: 09 Feb 2023 13:41
DOI: 10.1063/1.475706


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