Specific induction of cell motility on laminin by alpha 7 integrin

Echtermeyer, Frank, Schöber, Stefan, Pöschl, Ernst, von der Mark, Helga and von der Mark, Klaus (1996) Specific induction of cell motility on laminin by alpha 7 integrin. Journal of Biological Chemistry, 271 (4). pp. 2071-5. ISSN 0021-9258

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Laminin, the major glycoprotein of basement membranes, actively supports cell migration in development, tissue repair, tumor growth, metastasis, and other pathological processes. Previously we have shown that the locomotion of murine skeletal myoblasts is specifically and significantly enhanced on laminin but not on other matrix proteins. One of the major laminin receptors of myoblasts is the alpha 7 beta 1 integrin, which was first described in human MeWo melanoma cells and Rugli glioblastoma cells. In order to investigate and directly test the role of the alpha 7 integrin in cell migration on laminin, we expressed the murine alpha 7B splice variant in human 293 kidney cells and 530 melanoma cells which cannot migrate on laminin and are devoid of endogenous alpha 7. Northern blotting of the transfected cells showed that the alpha 7 mRNA was expressed efficiently, and the protein was detected on the cell surface by immunofluorescence and fluorescence-activated cell sorter analysis. Cell motility measurements by computer-assisted time-lapse videomicroscopy of the alpha 7-transfected cells revealed an 8-10-fold increase in motility on laminin-1 and its E8 fragment, but not on fibronectin. Mock-transfected cells did not migrate significantly of alpha 7-transfected 293 cells through laminin-coated filters in a Boyden chamber assay was significantly enhanced in comparison to mock transfected cells. These findings prove that alpha 7 integrin expression confers a gain of function-motile phenotype to immobile cells and may be responsible for transduction of the laminin-induced cell motility.

Item Type: Article
Uncontrolled Keywords: animals,antigens, cd,base sequence,cell adhesion,cell movement,cells, cultured,humans,integrin alpha chains,laminin,mice,molecular sequence data,recombinant proteins,video recording
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Cells and Tissues
Depositing User: Pure Connector
Date Deposited: 13 Jan 2016 12:02
Last Modified: 19 Apr 2023 23:45
URI: https://ueaeprints.uea.ac.uk/id/eprint/56293
DOI: 10.1074/jbc.271.4.2071

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