Cooperative and competitive interactions of regulatory elements are involved in the control of divergent transcription of human Col4A1 and Col4A2 genes

Pollner, Reinhold, Schmidt, Cornelia, Fischer, Gudrun, Kühn, Klaus and Pöschl, Ernst (1997) Cooperative and competitive interactions of regulatory elements are involved in the control of divergent transcription of human Col4A1 and Col4A2 genes. FEBS Letters, 405 (1). pp. 31-36. ISSN 0014-5793

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Abstract

The genes COL4A1 and COL4A2, coding for the two subunit chains alpha1(IV) and alpha2(IV) of collagen IV [alpha1(IV)2alpha2(IV)] are found closely linked on the human chromosome 13 in a unique head-to-head arrangement resulting in opposite strand transcription starting from a shared promoter region. Transient transfection experiments defined a shared promoter and two symmetrically arranged, downstream located and gene-specific activating elements in each gene. The shared promoter does not exhibit any transcriptional activity and efficient transcription depends on the cooperative effect of downstream elements. Mutual inhibitory effects between the two activating elements indicate competitive interactions with the shared promoter. Symmetry, cooperativity and competitivity of cis-elements are also reflected by the binding of transacting factors to the promoter and activating elements. From these data we propose a model for the coordination of divergent transcription of COL4 genes based on the cooperative and competitive interactions of the shared promoter and gene-specific regulating elements.

Item Type: Article
Uncontrolled Keywords: binding, competitive,collagen,gene expression regulation,humans,peptide chain initiation, translational,promoter regions, genetic,transcription, genetic,transcriptional activation,tumor cells, cultured
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 13 Jan 2016 12:01
Last Modified: 22 Apr 2020 00:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/56288
DOI: 10.1016/S0014-5793(97)00152-X

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