A diatom ferritin optimized for iron oxidation but not iron storage
Pfaffen, Stephanie, Bradley, Justin, Abdulqadir, Raz, Firme, Marlo R., Moore, Geoff, Le Brun, Nick and Murphy, Michael (2015) A diatom ferritin optimized for iron oxidation but not iron storage. The Journal of Biological Chemistry, 290. pp. 28416-28427. ISSN 1083-351X
![]() |
PDF (JBC_Diatom_ferritin_revised_manuscript_submitted)
- Submitted Version
Restricted to Repository staff only until 31 December 2099. Download (2MB) | Request a copy |
Abstract
Ferritin from the marine pennate diatom Pseudo-nitzschia multiseries (PmFTN) plays a key role in sustaining growth in iron-limited ocean environments. The di-iron catalytic ferroxidase center of PmFTN (sites A and B) has a nearby third iron site (site C) in an arrangement typically observed in prokaryotic ferritins. Here we demonstrate that Glu44, a site C ligand, and Glu130, a residue that bridges iron bound at sites B and C, limit the rate of post-oxidation reorganization of iron coordination and the rate at which Fe3+ exits the ferroxidase center for storage within the mineral core. The latter, in particular, severely limits the overall rate of iron mineralization. Thus, the diatom ferritin is optimized for initial Fe2+ oxidation but not for mineralization, pointing to a role for this protein in buffering iron availability and facilitating iron-sparing rather than only long-term iron storage.
Item Type: | Article |
---|---|
Faculty \ School: | Faculty of Science > School of Chemistry |
Depositing User: | Pure Connector |
Date Deposited: | 01 Dec 2015 07:27 |
Last Modified: | 18 Sep 2020 00:01 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/55534 |
DOI: | 10.1074/jbc.M115.669713 |
Actions (login required)
![]() |
View Item |