Different effect of Rho kinase inhibition on calcium signaling in rat isolated large and small arteries.

Martinsen, Anneloes, Yerna, Xavier, Rath, Géraldine, Gomez, Elvira, Dessy, Chantal and Morel, Nicole (2012) Different effect of Rho kinase inhibition on calcium signaling in rat isolated large and small arteries. Journal of Vascular Research, 49 (6). pp. 522-533. ISSN 1018-1172

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Abstract

In addition to its role in the regulation of artery contraction, Rho kinase (ROCK) was reported to be involved in the cytosolic calcium response to vasoconstrictor agonists in rat aorta and superior mesenteric artery (SMA). However, it remains to be determined whether ROCK also contributes to calcium signaling in resistance arteries, which play a major role in blood pressure regulation. The investigation of the effect of ROCK inhibition on the calcium and contractile responses of rat resistance mesenteric artery (RMA), in comparison with aorta and SMA, indicated that the calcium response to noradrenaline was inhibited by the ROCK inhibitor Y-27632 in aorta and SMA but not in RMA. The effect of Y-27632 on the calcium signal was unaffected by cytochalasin-D. ROCK activation in noradrenaline-stimulated arteries was confirmed by the inhibition of myosin light chain phosphorylation by Y-27632. Moreover, noradrenaline-induced calcium signaling was similarly inhibited by nimodipine in aorta, SMA and RMA, but nimodipine sensitivity of the contraction increased from the aorta to the RMA, suggesting that the contraction was controlled by different sources of calcium. In pressurized RMA, Y-27632 and H-1152 depressed pressure-induced calcium responses and abolished myogenic contraction. These results stress the important differences in calcium signaling between conductance and resistance arteries.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 18 Sep 2015 14:52
Last Modified: 22 Apr 2020 08:49
URI: https://ueaeprints.uea.ac.uk/id/eprint/54350
DOI: 10.1159/000341230

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