Three Pseudomonas putida FNR family proteins with different sensitivities to O2

Ibrahim, Susan A., Crack, Jason C., Rolfe, Matthew D., Borrero-de Acuňa, Jose Manuel, Thomson, Andrew J., Le Brun, Nick E. ORCID:, Schobert, Max, Stapleton, Melanie R. and Green, Jeffrey (2015) Three Pseudomonas putida FNR family proteins with different sensitivities to O2. The Journal of Biological Chemistry, 290. pp. 16812-16823. ISSN 1083-351X

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The Escherichia coli Fumarate-Nitrate Reduction regulator (FNR) protein is the paradigm for bacterial O2-sensing transcription factors. However, unlike E. coli, some bacterial species possess multiple FNR proteins that presumably have evolved to fulfill distinct roles. Here, three FNR proteins (ANR, PP_3233 and PP_3287) from a single bacterial species, Pseudomonas putida KT2440, have been analyzed. Under anaerobic conditions, all three proteins had spectral properties resembling those of [4Fe-4S] proteins. The reactivity of the ANR [4Fe-4S] cluster with O2 was similar to that of E. coli FNR and during conversion to the apo-protein, via a [2Fe-2S] intermediate, cluster sulfur was retained. Like ANR, reconstituted PP_3233 and PP_3287 were converted to [2Fe-2S] forms when exposed to O2, but their [4Fe-4S] clusters reacted more slowly. Transcription from an FNR-dependent promoter with a consensus FNR-binding site in P. putida and E. coli strains expressing only one FNR protein was consistent with the in vitro responses to O2. Taken together the experimental results suggest that the local environments of the iron-sulfur clusters in the different P. putida FNR proteins influence their reactivity with O2, such that ANR resembles E. coli FNR and is highly-responsive to low concentrations of O2, whereas PP_3233 and PP_3287 have evolved to be less sensitive to O2.

Item Type: Article
Additional Information: Free via Creative Commons: CC-BY license Copyright © 2015, The American Society for Biochemistry and Molecular Biology.
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Depositing User: Pure Connector
Date Deposited: 27 Aug 2015 14:54
Last Modified: 04 Jul 2023 09:15
DOI: 10.1074/jbc.M115.654079


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