Characterisation of osteoprotegerin autoantibodies in coeliac disease

Real, Ana, Gilbert, Nick, Hauser, Barbara, Kennedy, Nick, Shand, Alan, Gillett, Helen, Gillett, Peter, Goddard, Clive, Cebolla, Ángel, Sousa, Carolina, Fraser, William, Satsangi, Jack, Ralston, Stuart and Riches, Philip (2015) Characterisation of osteoprotegerin autoantibodies in coeliac disease. Calcified Tissue International, 97 (2). pp. 125-133. ISSN 0171-967X

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Objectives: Autoantibodies neutralising the effect of the bone regulatory cytokine osteoprotegerin (OPG) have been described in a patient with severe osteoporosis and coeliac disease. This study aimed to determine the prevalence and epitope specificity of autoantibodies to OPG in patients with coeliac disease, and correlate their presence with bone mineral density.   Methods: A direct enzyme linked immunosorbent assay was developed and used to screen patients with coeliac disease for autoantibodies to OPG. Recombinant fragments of OPG were made to evaluate the epitope specificity and affinity of these antibodies. Phenotype information of the patients was obtained by case note review.   Results: Raised titres of antibodies to OPG were found in 7/71 (9.8%) patients with coeliac disease, compared with 1/72 (1.4%) non-coeliac osteoporosis clinic control patients (p<0.05). Our results suggest a polyclonal antibody response to OPG is raised in these patients capable of recognising different epitopes of OPG with varying affinity. The titre of OPG antibodies was associated with lower bone mineral density Z score of the hip in coeliac patients on univariate (p<0.05) and multivariate analysis including age, sex height and weight as covariates (p<0.01).   Conclusion: Polyclonal antibodies to OPG are more common in patients with coeliac disease and are independently associated with lower bone mineral density Z scores of the hip. Further work is required to establish the clinical utility of testing for OPG antibodies.

Item Type: Article
Uncontrolled Keywords: osteoporosis,coeliac disease,osteoprotegerin,autoantibodies
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 23:08
Last Modified: 19 Oct 2023 01:28
DOI: 10.1007/s00223-015-0023-4

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