Diagnostic and clinical significance of Crohn’s disease specific pancreatic anti-GP2 and anti-CUZD1 antibodies

Pavlidis, Polychronos, Forbes, Alastair ORCID: https://orcid.org/0000-0001-7416-9843 and Bogdanos, Dimitrios P (2015) Diagnostic and clinical significance of Crohn’s disease specific pancreatic anti-GP2 and anti-CUZD1 antibodies. Clinical Chemistry and Laboratory Medicine, 441. 176–181. ISSN 1434-6621

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Background: Pancreatic autoantibodies (PAB) targeting GP2 and CUZD1 are Crohn’s disease (CrD)-markers. The clinical significance of anti-GP2 antibodies has been assessed, but that of anti-CUZD1 remains elusive. Aim: To assess the clinical utility of anti-CUZD1/anti-GP2 by novel cell-based indirect immunofluorescence (IIF) assays in CrD. Methods: A total of 212 CrD and 249 UC patients followed-up at a London IBD centre were investigated to simultaneously detect PABs, anti-GP2 and anti-CUZD1 by IIF using primate pancreatic tissue, and HEK293 over-expressing CUZD1 or GP2. Results: Overall, 88 (41.5%) CrDs compared to 26 (10.4%) UCs (p<0.001) tested positive for IgA and/or IgG anti-GP2 and/or anti-CUZD1 antibodies, while ASCA were found in 67.5% CrDs vs 19.2% UCs (p<0.0001); ASCA and/or PAB (anti-GP2 or anti-CUZD1) were detected in 76% CrD vs 34% UC patients. IgG anti-GP2 antibodies were less prevalent in L2 phenotype (p=0.002) and more prevalent in patients with stricturing disease (p=0.0418), even when a higher cut off (≥1,000 RU) was used (p=0.0396). Also, anti-GP2 IgG positive CrD patients had younger age of disease onset. IgA and/or IgG ASCA & anti-GP2 IgG antibody positive CrDs had younger onset of disease (p<0.0001), were more likely to have both ileal and colonic disease (p<0.0001) and had more stricturing (p <0.0001) than seronegative patients. Clinical correlates were not found for anti-CUZD1 positivity. Conclusions: PAB testing increases ASCA’s serological sensitivity for CrD. Anti-GP2 detection, in isolation or in combination with ASCA, stratify CrD patients which phenotypically are characterised by a much younger onset of disease, extensive and stricturing behaviour.

Item Type: Article
Uncontrolled Keywords: autoantibody,bowel disease,marker,sensitivity,specificity
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine
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Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 23:04
Last Modified: 21 Oct 2022 01:03
URI: https://ueaeprints.uea.ac.uk/id/eprint/53807
DOI: 10.1016/j.cca.2014.12.010

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