The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

Azeem, Ayesha, English, Andrew, Kumar, Pramod, Satyam, Abhigyan, Biggs, Manus, Jones, Eleanor, Tripathi, Bhawana, Basu, Nandita, Henkel, Jan, Vaquette, Cedryck, Rooney, Niall, Riley, Graham ORCID:, O'Riordan, Alan, Cross, Graham, Ivanovski, Saso, Hutmacher, Dietmar, Pandit, Abhay and Zeugolis, Dimitrios (2015) The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis. Nanomedicine, 10 (5). pp. 693-711. ISSN 1743-5889

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AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface. MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed. RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo. CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

Item Type: Article
Uncontrolled Keywords: anisotropic topography,biomaterial interface,gene analysis,osteogenesis,mesenchymal stem-cells,tissue engineering applications,peripheral-nerve repair,focal adhesion kinase,regenerative medicine,drug-delivery,osteoblast alignment,controlled-release,soft-lithography,cellular mechanotransduction
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine
Faculty of Science > Research Groups > Cells and Tissues
Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 22:25
Last Modified: 04 Sep 2023 00:18
DOI: 10.2217/nnm.14.218

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