The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

Azeem, Ayesha, English, Andrew, Kumar, Pramod, Satyam, Abhigyan, Biggs, Manus, Jones, Eleanor, Tripathi, Bhawana, Basu, Nandita, Henkel, Jan, Vaquette, Cedryck, Rooney, Niall, Riley, Graham, O'Riordan, Alan, Cross, Graham, Ivanovski, Saso, Hutmacher, Dietmar, Pandit, Abhay and Zeugolis, Dimitrios (2015) The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis. Nanomedicine, 10 (5). pp. 693-711. ISSN 1743-5889

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Abstract

AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface. MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed. RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo. CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

Item Type: Article
Uncontrolled Keywords: anisotropic topography,biomaterial interface,gene analysis,osteogenesis,mesenchymal stem-cells,tissue engineering applications,peripheral-nerve repair,focal adhesion kinase,regenerative medicine,drug-delivery,osteoblast alignment,controlled-release,soft-lithography,cellular mechanotransduction
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 22:25
Last Modified: 22 Apr 2020 00:08
URI: https://ueaeprints.uea.ac.uk/id/eprint/53313
DOI: 10.2217/nnm.14.218

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