Matrix metalloproteinase-9 maps to the distal end of chromosome 2 in the mouse

Leco, Kevin J., Harvey, Mark B., Hogan, Aileen, Copeland, Neal G., Gilbert, Debra J., Jenkins, Nancy A., Edwards, Dylan R. ORCID: and Schultz, Gilbert A. (1997) Matrix metalloproteinase-9 maps to the distal end of chromosome 2 in the mouse. Developmental Genetics, 21 (1). pp. 55-60. ISSN 0192-253X

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The activity and expression of matrix metalloproteinase-9/gelatinase B (MMP-9), an enzyme implicated in the implantation process in mice, was investigated in normal and parthenogenetic blastocyst outgrowths. Conditioned media from parthenogenetic blastocysts after 4 days of culture had reduced levels of MMP-9 activity compared to conditioned medium from normal outgrowths. Levels of MMP-9 mRNA assayed by reverse transcription-polymerase chain reaction methods were also reduced in parthenogenetic blastocysts compared to normal outgrowths. Genetic mapping studies showed that Mmp9 maps to the distal end of chromosome 2 near the proximal boundary of a region affected by genomic imprinting. Both parental alleles of Mmp9, however, are expressed in 11.5-day embryos derived from interspecific crosses of Mus musculus and Mus spretus. Thus, loss of MMP-9 activity in parthenogenetic blastocysts does not appear to be due to imprinting but, rather, due to a defect of trophoblast giant cell proliferation and differentiation.

Item Type: Article
Uncontrolled Keywords: animals,blastocyst,chromosome mapping,chromosomes,collagenases,matrix metalloproteinase 9,mice,parthenogenesis
Faculty \ School: Faculty of Science > The Sainsbury Laboratory
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Cells and Tissues
Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
Depositing User: Pure Connector
Date Deposited: 25 Mar 2015 18:23
Last Modified: 19 Apr 2023 00:37
DOI: 10.1002/(SICI)1520-6408(1997)21:1<55::AID-DVG6>3.0.CO;2-7

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