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Leco, Kevin J., Harvey, Mark B., Hogan, Aileen, Copeland, Neal G., Gilbert, Debra J., Jenkins, Nancy A., Edwards, Dylan R. 
ORCID: https://orcid.org/0000-0002-3292-2064 and Schultz, Gilbert A.
(1997)
Matrix metalloproteinase-9 maps to the distal end of chromosome 2 in the mouse.
Developmental Genetics, 21 (1).
pp. 55-60.
ISSN 0192-253X
Abstract
The activity and expression of matrix metalloproteinase-9/gelatinase B (MMP-9), an enzyme implicated in the implantation process in mice, was investigated in normal and parthenogenetic blastocyst outgrowths. Conditioned media from parthenogenetic blastocysts after 4 days of culture had reduced levels of MMP-9 activity compared to conditioned medium from normal outgrowths. Levels of MMP-9 mRNA assayed by reverse transcription-polymerase chain reaction methods were also reduced in parthenogenetic blastocysts compared to normal outgrowths. Genetic mapping studies showed that Mmp9 maps to the distal end of chromosome 2 near the proximal boundary of a region affected by genomic imprinting. Both parental alleles of Mmp9, however, are expressed in 11.5-day embryos derived from interspecific crosses of Mus musculus and Mus spretus. Thus, loss of MMP-9 activity in parthenogenetic blastocysts does not appear to be due to imprinting but, rather, due to a defect of trophoblast giant cell proliferation and differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | animals,blastocyst,chromosome mapping,chromosomes,collagenases,matrix metalloproteinase 9,mice,parthenogenesis |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies |
| Depositing User: | Pure Connector |
| Date Deposited: | 25 Mar 2015 18:23 |
| Last Modified: | 19 Apr 2023 00:37 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/52963 |
| DOI: | 10.1002/(SICI)1520-6408(1997)21:1<55::AID-DVG6>3.0.CO;2-7 |
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