Tools
ToolsGu, Yinghong, Stansfeld, Phillip J., Zeng, Yi, Dong, Haohao, Wang, Wenjian and Dong, Changjiang (2015) Lipopolysaccharide is inserted into the outer membrane through an intramembrane hole, a lumen gate, and the lateral opening of LptD. Structure, 23 (3). 496–504. ISSN 0969-2126
Preview |
PDF (Published manuscript)
- Published Version
Available under License Creative Commons Attribution. Download (3MB) | Preview |
Abstract
Lipopolysaccharide (LPS) is essential for the vitality of most Gram-negative bacteria and plays an important role in bacterial multidrug resistance. The LptD/E translocon inserts LPS into the outer leaflet, the mechanism of which is poorly understood. Here, we report mutagenesis, functional assays, and molecular dynamics simulations of the LptD/E complex, which suggest two distinct pathways for the insertion of LPS. The N-terminal domain of LptD comprises a hydrophobic slide that injects the acyl tails of LPS directly into the outer membrane through an intramembrane hole, while the core oligosaccharide and O-antigen pass a lumen gate that triggers the unzipping of the lateral opening between strands β1C and β26C of the barrel of LptD, to finalize LPS insertion. Mutation of the LPS transport related residues or block of the LPS transport pathways results in the deaths of Escherichia coli. These findings are important for the development of novel antibiotics.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Depositing User: | Pure Connector |
| Date Deposited: | 24 Mar 2015 16:12 |
| Last Modified: | 19 Aug 2023 00:16 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/52853 |
| DOI: | 10.1016/j.str.2015.01.001 |
Actions (login required)
 |
View Item |