Biophysical and electrochemical studies of protein-nucleic acid interactions

Bowater, Richard ORCID:, Cobb, Andrew, Pivonkova, Hana, Havran, Ludek and Fojta, Miroslav (2015) Biophysical and electrochemical studies of protein-nucleic acid interactions. Monatshefte für Chemie - Chemical Monthly, 146 (5). pp. 723-739. ISSN 0026-9247

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This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail.

Item Type: Article
Uncontrolled Keywords: bioorganic chemistry,biosensors,electrochemistry,electron transfer,electrophoresis,nucleic acids,proteins,voltammetry,biochemistry, genetics and molecular biology (miscellaneous),biophysics,electrochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1301
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Biosciences Teaching and Education Research
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Depositing User: Pure Connector
Date Deposited: 16 Feb 2015 12:10
Last Modified: 19 Apr 2023 00:28
DOI: 10.1007/s00706-014-1405-4


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