Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling

Hemmerling, J, Jansen, J, Müller, M ORCID: https://orcid.org/0000-0002-5930-9905 and Haller, D (2015) Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling. Journal of Immunological Methods, 416. 189–192. ISSN 0022-1759

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Abstract

Laser microdissection (LMD) technology enables highly specific gene expression analyses of biologically relevant questions at cell- or tissue-specific resolution. Nevertheless, specific cell types are often limited in quantity (i.e. fetal tissue), making high quality RNA extraction and subsequent gene expression approaches via common reverse transcriptase-quantitative PCR (RT-q-PCR) challenging. In the case of fetal gut epithelia representing immune modulatory interphases gene expression analysis with common RT-q-PCR is limited to a few genes (

Item Type:	Article
Additional Information:	Copyright © 2014 Elsevier B.V. All rights reserved.
Uncontrolled Keywords:	fetal intestinal epithelium,laser microdissection,whole transcriptome preamplification
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	28 Jan 2015 12:36
Last Modified:	18 Jun 2026 16:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/52030
DOI:	10.1016/j.jim.2014.11.008

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