Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling

Hemmerling, J, Jansen, J, Müller, M and Haller, D (2015) Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling. Journal of Immunological Methods, 416. 189–192. ISSN 0022-1759

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Abstract

Laser microdissection (LMD) technology enables highly specific gene expression analyses of biologically relevant questions at cell- or tissue-specific resolution. Nevertheless, specific cell types are often limited in quantity (i.e. fetal tissue), making high quality RNA extraction and subsequent gene expression approaches via common reverse transcriptase-quantitative PCR (RT-q-PCR) challenging. In the case of fetal gut epithelia representing immune modulatory interphases gene expression analysis with common RT-q-PCR is limited to a few genes (

Item Type: Article
Additional Information: Copyright © 2014 Elsevier B.V. All rights reserved.
Uncontrolled Keywords: fetal intestinal epithelium,laser microdissection,whole transcriptome preamplification
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 28 Jan 2015 12:36
Last Modified: 21 Apr 2020 23:49
URI: https://ueaeprints.uea.ac.uk/id/eprint/52030
DOI: 10.1016/j.jim.2014.11.008

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