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Hemmerling, J, Jansen, J, Müller, M 
ORCID: https://orcid.org/0000-0002-5930-9905 and Haller, D
(2015)
Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling.
Journal of Immunological Methods, 416.
189–192.
ISSN 0022-1759
Abstract
Laser microdissection (LMD) technology enables highly specific gene expression analyses of biologically relevant questions at cell- or tissue-specific resolution. Nevertheless, specific cell types are often limited in quantity (i.e. fetal tissue), making high quality RNA extraction and subsequent gene expression approaches via common reverse transcriptase-quantitative PCR (RT-q-PCR) challenging. In the case of fetal gut epithelia representing immune modulatory interphases gene expression analysis with common RT-q-PCR is limited to a few genes (
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright © 2014 Elsevier B.V. All rights reserved. |
| Uncontrolled Keywords: | fetal intestinal epithelium,laser microdissection,whole transcriptome preamplification |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Depositing User: | Pure Connector |
| Date Deposited: | 28 Jan 2015 12:36 |
| Last Modified: | 21 Oct 2022 00:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/52030 |
| DOI: | 10.1016/j.jim.2014.11.008 |
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