Comparative analysis of ESBL-positive Escherichia coli isolates from animals and humans from the UK, The Netherlands and Germany

Wu, Guanghui, Day, Michaela J, Mafura, Muriel T, Nunez-Garcia, Javier, Fenner, Jackie J, Sharma, Meenaxi, van Essen-Zandbergen, Alieda, Rodríguez, Irene, Dierikx, Cindy, Kadlec, Kristina, Schink, Anne-Kathrin, Wain, John, Helmuth, Reiner, Guerra, Beatriz, Schwarz, Stefan, Threlfall, John, Woodward, Martin J, Woodford, Neil, Coldham, Nick and Mevius, Dik (2013) Comparative analysis of ESBL-positive Escherichia coli isolates from animals and humans from the UK, The Netherlands and Germany. PLoS One, 8 (9). ISSN 1932-6203

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Abstract

The putative virulence and antimicrobial resistance gene contents of extended spectrum β-lactamase (ESBL)-positive E. coli (n=629) isolated between 2005 and 2009 from humans, animals and animal food products in Germany, The Netherlands and the UK were compared using a microarray approach to test the suitability of this approach with regard to determining their similarities. A selection of isolates (n=313) were also analysed by multilocus sequence typing (MLST). Isolates harbouring bla(CTX-M-group-1) dominated (66%, n=418) and originated from both animals and cases of human infections in all three countries; 23% (n=144) of all isolates contained both bla(CTX-M-group-1) and bla(OXA-1-like) genes, predominantly from humans (n=127) and UK cattle (n=15). The antimicrobial resistance and virulence gene profiles of this collection of isolates were highly diverse. A substantial number of human isolates (32%, n=87) did not share more than 40% similarity (based on the Jaccard coefficient) with animal isolates. A further 43% of human isolates from the three countries (n=117) were at least 40% similar to each other and to five isolates from UK cattle and one each from Dutch chicken meat and a German dog; the members of this group usually harboured genes such as mph(A), mrx, aac(6')-Ib, catB3, bla(OXA-1-like) and bla(CTX-M-group-1). forty-four per cent of the MLST-typed isolates in this group belonged to ST131 (n=18) and 22% to ST405 (n=9), all from humans. Among animal isolates subjected to MLST (n=258), only 1.2% (n=3) were more than 70% similar to human isolates in gene profiles and shared the same MLST clonal complex with the corresponding human isolates. The results suggest that minimising human-to-human transmission is essential to control the spread of ESBL-positive E. coli in humans.

Item Type: Article
Additional Information: Correction: 17 Sep 2014: The PLOS ONE Staff (2014) Correction: Comparative Analysis of ESBL-Positive Escherichia coli Isolates from Animals and Humans from the UK, The Netherlands and Germany. PLoS ONE 9(9): e108834. doi: 10.1371/journal.pone.0108834 © 2013 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Uncontrolled Keywords: animal feed,animals,cattle,chickens,dogs,drug resistance,escherichia coli,germany,great britain,humans,microarray analysis,multilocus sequence typing,netherlands,species specificity,virulence,beta-lactamases
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 12 Nov 2014 16:48
Last Modified: 21 Apr 2020 23:33
URI: https://ueaeprints.uea.ac.uk/id/eprint/50755
DOI: 10.1371/journal.pone.0075392

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