Cross-species comparison of genes related to nutrient sensing mechanisms expressed along the intestine

van der Wielen, Nikkie, van Avesaat, Mark, de Wit, Nicole J. W., Vogels, Jack T. W. E., Troost, Freddy, Masclee, Ad, Koopmans, Sietse-Jan, van der Meulen, Jan, Boekschoten, Mark V., Müller, Michael ORCID:, Hendriks, Henk F. J., Witkamp, Renger F. and Meijerink, Jocelijn (2014) Cross-species comparison of genes related to nutrient sensing mechanisms expressed along the intestine. PLoS One, 9 (9). ISSN 1932-6203

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Introduction: Intestinal chemosensory receptors and transporters are able to detect food-derived molecules and are involved in the modulation of gut hormone release. Gut hormones play an important role in the regulation of food intake and the control of gastrointestinal functioning. This mechanism is often referred to as “nutrient sensing”. Knowledge of the distribution of chemosensors along the intestinal tract is important to gain insight in nutrient detection and sensing, both pivotal processes for the regulation of food intake. However, most knowledge is derived from rodents, whereas studies in man and pig are limited, and cross-species comparisons are lacking. Aim: To characterize and compare intestinal expression patterns of genes related to nutrient sensing in mice, pigs and humans. Methods: Mucosal biopsy samples taken at six locations in human intestine (n = 40) were analyzed by qPCR. Intestinal scrapings from 14 locations in pigs (n = 6) and from 10 locations in mice (n = 4) were analyzed by qPCR and microarray, respectively. The gene expression of glucagon, cholecystokinin, peptide YY, glucagon-like peptide-1 receptor, taste receptor T1R3, sodium/glucose cotransporter, peptide transporter-1, GPR120, taste receptor T1R1, GPR119 and GPR93 was investigated. Partial least squares (PLS) modeling was used to compare the intestinal expression pattern between the three species. Results and conclusion: The studied genes were found to display specific expression patterns along the intestinal tract. PLS analysis showed a high similarity between human, pig and mouse in the expression of genes related to nutrient sensing in the distal ileum, and between human and pig in the colon. The gene expression pattern was most deviating between the species in the proximal intestine. Our results give new insights in interspecies similarities and provide new leads for translational research and models aiming to modulate food intake processes in man.

Item Type: Article
Additional Information: © 2014 van der Wielen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine
Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User: Pure Connector
Date Deposited: 08 Oct 2014 08:46
Last Modified: 06 Jun 2024 14:50
DOI: 10.1371/journal.pone.0107531


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