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Tezuka, A, Kasagi, S, Van Oosterhout, C 
ORCID: https://orcid.org/0000-0002-5653-738X, Mcmullan, M, Iwasaki, W M, Kasai, D, Yamamichi, M, Innan, H, Kawamura, S and Kawata, M
(2014)
Divergent selection for opsin gene variation in guppy (Poecilia reticulata) populations of Trinidad and Tobago.
Heredity, 113.
pp. 381-389.
ISSN 0018-067X
Abstract
The guppy is known to exhibit remarkable interindividual variations in spectral sensitivity of middle to long wavelength-sensitive (M/LWS) cone photoreceptor cells. The guppy has four M/LWS-type opsin genes (LWS-1, LWS-2, LWS-3 and LWS-4) that are considered to be responsible for this sensory variation. However, the allelic variation of the opsin genes, particularly in terms of their absorption spectrum, has not been explored in wild populations. Thus, we examined nucleotide variations in the four M/LWS opsin genes as well as blue-sensitive SWS2-B and ultraviolet-sensitive SWS1 opsin genes for comparison and seven non-opsin nuclear loci as reference genes in 10 guppy populations from various light environments in Trinidad and Tobago. For the first time, we discovered a potential spectral variation (180 Ser/Ala) in LWS-1 that differed at an amino acid site known to affect the absorption spectra of opsins. Based on a coalescent simulation of the nucleotide variation of the reference genes, we showed that the interpopulation genetic differentiation of two opsin genes was significantly larger than the neutral expectation. Furthermore, this genetic differentiation was significantly related to differences in dissolved oxygen (DO) level, and it was not explained by the spatial distance between populations. The DO levels are correlated with eutrophication that possibly affects the color of aquatic environments. These results suggest that the population diversity of opsin genes is significantly driven by natural selection and that the guppy could adapt to various light environments through color vision changes.
| Item Type: | Article |
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| Additional Information: | Acknowledgements: The authors thank J Cable for providing the guppy fins, A Shoji, J Yokoyama and N Matsushima for determining the opsin gene sequences, T Makino for designing the primers for the reference genes and Y Terai for commenting on the manuscript. MK was supported by a Grant-in-Aid for Scientific Research (21370007) from the Japan Society for the Promotion of Science (JSPS) and partly by the Global COE Program ‘Centre for ecosystem management adapting to global change’ (J03) of the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). SK was supported by a Grant-in-Aid for Scientific Research A (22247036), a Grant-in-Aid for Challenging Exploratory Research (23657164) from JSPS and Grants-in-Aid for Scientific Research on Priority Areas ‘Comparative Genomics’ (20017008) and ‘Cellular Sensor’ (21026007) from MEXT. MY was supported by a Research Fellowship from the Japan Society for the Promotion of Science (JSPS) for Young Scientists (21-7611). CvO and MM were funded by the Earth & Life Systems Alliance (ELSA). The authors thank M Hattori and K Oshima for support with nucleotide sequencing through a Grant-in-Aid for Scientific Research on Innovative Areas ‘Genome Science’ from Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 221S0002). |
| Faculty \ School: | Faculty of Science > School of Environmental Sciences |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation |
| Depositing User: | Pure Connector |
| Date Deposited: | 08 Oct 2014 08:50 |
| Last Modified: | 23 Jul 2024 00:39 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/50449 |
| DOI: | 10.1038/hdy.2014.35 |
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