Map positions of 47 Arabidopsis sequences with sequence similarity to disease resistance genes

Botella, Miguel A., Coleman, Mark J., Hughes, Douglas E., Nishimura, Marc T., Jones, Jonathan D. G. and Somerville, Shauna C. (1997) Map positions of 47 Arabidopsis sequences with sequence similarity to disease resistance genes. The Plant Journal, 12 (5). pp. 1197-1211. ISSN 0960-7412

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Map positions have been determined for 42 non-redundant Arabidopsis expressed sequence tags (ESTs) showing similarity to disease resistance genes (R-ESTs), and for three Pto-like sequences that were amplified with degenerate primers. Employing a PCR-based strategy, yeast artificial chromosome (YAC) clones containing the EST sequences were identified. Since many YACs have been mapped, the locations of the R-ESTs could be inferred from the map positions of the YACs. R-EST clones that exhibited ambiguous map positions were mapped as either cleavable amplifiable polymorphic sequence (CAPS) or restriction fragment length polymorphism (RFLP) markers using F8 (Ler x Col-0) recombinant inbred (RI) lines. In all cases but two, the R-ESTs and Pto-like sequences mapped to single, unique locations. One R-EST and one Pto-like sequence each mapped to two locations. Thus, a total of 47 loci were identified in this study. Several R-ESTs occur in clusters suggesting that they may have arisen via gene duplication events. Interestingly, several R-ESTs map to regions containing genetically defined disease resistance genes. Thus, this collection of mapped R-ESTs may expedite the isolation of disease resistance genes. As the cDNA sequencing projects have identified an estimated 63% of Arabidopsis genes, a very large number of R-ESTs (∼95), and by inference disease resistance genes of the leucine-rich repeat-class probably occur in the Arabidopsis genome.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
Depositing User: Pure Connector
Date Deposited: 06 Oct 2014 15:58
Last Modified: 21 Oct 2022 00:11
DOI: 10.1046/j.1365-313X.1997.12051197.x

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