Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

Xu, Ping, Mohorianu, Irina, Yang, Li, Zhao, Hansheng, Gao, Zhimin and Dalmay, Tamas ORCID: (2014) Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters. PLoS One, 9 (7). ISSN 1932-6203

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Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.

Item Type: Article
Additional Information: © 2014 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
Depositing User: Pure Connector
Date Deposited: 21 Aug 2014 08:44
Last Modified: 31 Oct 2023 01:44
DOI: 10.1371/journal.pone.0103590


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