The measurement of exchangeable pools of zinc using the stable isotope 70Zn

Fairweather-Tait, Susan J. ORCID:, Jackson, Malcolm J., Fox, Thomas E., Wharf, S. Gabrielle, Eagles, John and Croghan, Peter C. (1993) The measurement of exchangeable pools of zinc using the stable isotope 70Zn. British Journal of Nutrition, 70 (1). pp. 221-234. ISSN 0007-1145

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The present study was designed to assess the feasibility of using small doses of a stable isotope of Zn to follow plasma kinetics over a 10 d period and, hence, make deductions about Zn turnover and body pool sizes. At the beginning of the 10 d metabolic balance, two adults, consuming their habitual diet, were given an intravenous injection of 70Zn. There was a fourfold difference in the administered dose between the two subjects (0.445 and 2.078 mg). Blood samples were taken at regular intervals and plasma enrichment with 70Zn measured by thermal ionization mass spectrometry. Urine and faeces were collected and analysed for Zn and 70Zn. Kinetic analysis of the plasma 70Zn decay by several different methods was undertaken. It was apparent from both deconvolution analysis of the short-term (0-90 min) decay data and four-compartment modelling of the longer-term (0-24 h) data that isotopic Zn very rapidly equilibrates with the plasma Zn and with a rapidly exchanging non-plasma pool, probably located within the liver. This latter pool appears to contain less than 10 mg Zn and the peak of isotope enrichment occurs at about 20 min post injection. The later decay of plasma Zn enrichment appears to be dictated by exchange with a much larger pool of approximate size 350 mg.

Item Type: Article
Uncontrolled Keywords: adult,dietary proteins,energy intake,female,humans,kinetics,male,mathematics,models, biological,zinc,zinc isotopes
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine
Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine
Depositing User: Pure Connector
Date Deposited: 05 Aug 2014 12:48
Last Modified: 24 Oct 2022 06:32
DOI: 10.1079/BJN19930119

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