The usefulness of in vitro models to predict the bioavailability of iron and zinc:a consensus statement from the HarvestPlus expert consultation

Fairweather-Tait, Sue, Lynch, Sean, Hotz, Christine, Hurrell, Richard, Abrahamse, Leo, Beebe, Steve, Bering, Stine, Bukhave, Klaus, Glahn, Ray, Hambidge, Michael, Hunt, Janet, Lonnerdal, Bo, Miller, Denis, Mohktar, Najat, Nestel, Penelope, Reddy, Manju, Sandber, Ann-Sofie, Sharp, Paul, Teucher, Birgit and Trinidad, Trinidad P (2005) The usefulness of in vitro models to predict the bioavailability of iron and zinc:a consensus statement from the HarvestPlus expert consultation. International Journal for Vitamin and Nutrition Research, 75 (6). pp. 371-374. ISSN 0300-9831

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Abstract

A combination of dietary and host-related factors determines iron and zinc absorption, and several in vitro methods have been developed as preliminary screening tools for assessing bioavailability. An expert committee has reviewed evidence for their usefulness and reached a consensus. Dialyzability (with and without simulated digestion) gives some useful information but cannot predict the correct magnitude of response and may sometimes predict the wrong direction of response. Caco-2 cell systems (with and without simulated digestion) have been developed for iron availability, but the magnitude of different effects does not always agree with results obtained in human volunteers, and the data for zinc are too limited to draw conclusions about the validity of the method. Caco-2 methodologies vary significantly between laboratories and require experienced technicians and good quality cell culture facilities to obtain reproducible results. Algorithms can provide semi-quantitative information enabling diets to be classified as high, moderate, or low bioavailability. While in vitro methods can be used to generate ideas and develop hypotheses, they cannot be used alone for important decisions concerning food fortification policy, selection of varieties for plant breeding programs, or for new product development in the food industry. Ultimately human studies are required for such determinations.

Item Type: Article
Uncontrolled Keywords: biological availability,caco-2 cells,humans,iron,zinc
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 25 Jul 2014 12:58
Last Modified: 28 Oct 2019 13:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/49165
DOI: 10.1024/0300-9831.75.6.371

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