Crack, Jason C, Green, Jeffrey, Thomson, Andrew J and Le Brun, Nick E ORCID: https://orcid.org/0000-0001-9780-4061
(2014)
Techniques for the production, isolation, and analysis of iron-sulfur proteins.
Methods in Molecular Biology, 1122.
pp. 33-48.
ISSN 1064-3745
Abstract
Iron-sulfur clusters constitute a group of cofactors found in many proteins that play key roles in an exceptionally wide range of metabolic processes. The chemical reactivity of iron-sulfur clusters means that they can be particularly prone to damage when removed from the protective environment of the cell. In general, the key to obtaining an intact, biologically active iron-sulfur cluster-containing protein is to maintain a strictly anaerobic environment throughout the entire process of protein purification and analysis. For many proteins, particularly those with more labile clusters, it is essential.
Item Type: | Article |
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Faculty \ School: | Faculty of Science > School of Chemistry |
Depositing User: | Pure Connector |
Date Deposited: | 09 Jul 2014 10:10 |
Last Modified: | 24 Oct 2022 06:29 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/49064 |
DOI: | 10.1007/978-1-62703-794-5_4 |
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