Techniques for the production, isolation, and analysis of iron-sulfur proteins

Crack, Jason C, Green, Jeffrey, Thomson, Andrew J and Le Brun, Nick E ORCID: https://orcid.org/0000-0001-9780-4061 (2014) Techniques for the production, isolation, and analysis of iron-sulfur proteins. Methods in Molecular Biology, 1122. pp. 33-48. ISSN 1064-3745

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Abstract

Iron-sulfur clusters constitute a group of cofactors found in many proteins that play key roles in an exceptionally wide range of metabolic processes. The chemical reactivity of iron-sulfur clusters means that they can be particularly prone to damage when removed from the protective environment of the cell. In general, the key to obtaining an intact, biologically active iron-sulfur cluster-containing protein is to maintain a strictly anaerobic environment throughout the entire process of protein purification and analysis. For many proteins, particularly those with more labile clusters, it is essential.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes
Depositing User:	Pure Connector
Date Deposited:	09 Jul 2014 10:10
Last Modified:	24 Oct 2022 06:29
URI:	https://ueaeprints.uea.ac.uk/id/eprint/49064
DOI:	10.1007/978-1-62703-794-5_4

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