In vivo speciation studies and antioxidant properties of bromine in Laminaria digitata reinforce the significance of iodine accumulation for kelps

Küpper, Frithjof C., Carpenter, Lucy J., Leblanc, Catherine, Toyama, Chiaki, Uchida, Yuka, Maskrey, Benjamin H., Robinson, Joanne, Verhaeghe, Elodie F., Malin, Gill ORCID:, Luther, George W., Kroneck, Peter M. H., Kloareg, Bernard, Meyer-Klaucke, Wolfram, Muramatsu, Yasuyuki, Megson, Ian L., Potin, Philippe and Feiters, Martin C. (2013) In vivo speciation studies and antioxidant properties of bromine in Laminaria digitata reinforce the significance of iodine accumulation for kelps. Journal of Experimental Botany, 64 (10). pp. 2653-2664. ISSN 0022-0957

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The metabolism of bromine in marine brown algae remains poorly understood. This contrasts with the recent finding that the accumulation of iodide in the brown alga Laminaria serves the provision of an inorganic antioxidant - the first case documented from a living system. The aim of this study was to use an interdisciplinary array of techniques to study the chemical speciation, transformation, and function of bromine in Laminaria and to investigate the link between bromine and iodine metabolism, in particular in the antioxidant context. First, bromine and iodine levels in different Laminaria tissues were compared by inductively coupled plasma MS. Using in vivo X-ray absorption spectroscopy, it was found that, similarly to iodine, bromine is predominantly present in this alga in the form of bromide, albeit at lower concentrations, and that it shows similar behaviour upon oxidative stress. However, from a thermodynamic and kinetic standpoint, supported by in vitro and reconstituted in vivo assays, bromide is less suitable than iodide as an antioxidant against most reactive oxygen species except superoxide, possibly explaining why kelps prefer to accumulate iodide. This constitutes the first-ever study exploring the potential antioxidant function of bromide in a living system and other potential physiological roles. Given the tissue-specific differences observed in the content and speciation of bromine, it is concluded that the bromide uptake mechanism is different from the vanadium iodoperoxidase-mediated uptake of iodide in L. digitata and that its function is likely to be complementary to the iodide antioxidant system for detoxifying superoxide.

Item Type: Article
Uncontrolled Keywords: sdg 14 - life below water ,/dk/atira/pure/sustainabledevelopmentgoals/life_below_water
Faculty \ School: Faculty of Science > School of Environmental Sciences
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Faculty of Science > Research Groups > Environmental Biology
Faculty of Science > Research Groups > Collaborative Centre for Sustainable Use of the Seas
Faculty of Science > Research Groups > Marine Knowledge Exchange Network
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Depositing User: Pure Connector
Date Deposited: 09 Jun 2014 21:06
Last Modified: 24 Oct 2022 06:20
DOI: 10.1093/jxb/ert110

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