Expression and modulation of matrix metalloproteinase-2 and tissue inhibitors of metalloproteinases in human embryonic CNS stem cells

Frölichsthal-Schoeller, Paola, Vescovi, Angelo L., Krekoski, Craig A., Murphy, Gillian, Edwards, Dylan R. and Forsyth, Peter (1999) Expression and modulation of matrix metalloproteinase-2 and tissue inhibitors of metalloproteinases in human embryonic CNS stem cells. NeuroReport, 10 (2). pp. 345-351. ISSN 0959-4965

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Abstract

The expression and regulation of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) in neuroectodermal precursor cells is undocumented. We report the presence of MMP-2, but no MMP-9, and of all the four known TIMPs in neuroepithelial stem cells isolated from the human CNS. The expression of TIMP-1, TIMP-2 and TIMP-3 was unchanged following stem cells differentiation into neurons and glia. In contrast, while MMP-2 and TIMP-4 were localized to both stem and mature CNS cells, their levels of expression were substantially reduced in the latter. TIMP-4 showed a 23-fold reduction in media conditioned by differentiated cells compared with stem cell-conditioned media, reflecting a 6-fold decrease in mRNA expression. Interestingly, TIMP-4 also differed from the other TIMPs in that it was cell- associated in the stem cells, where this fraction remained unchanged upon differentiation. Hence, regulation of selective MMPs and TIMPs occurs during differentiation of human neural precursors suggesting that MMP-2 and TIMP-4 in particular may perform regulatory roles in the developing CNS.

Item Type: Article
Uncontrolled Keywords: human cns stem cells,matrix metalloproteinases,neural development,neural precursors,tissue inhibitors of metalloproteinases
Faculty \ School:
Faculty of Science > School of Biological Sciences
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Depositing User: Pure Connector
Date Deposited: 16 May 2014 11:36
Last Modified: 21 Apr 2020 23:00
URI: https://ueaeprints.uea.ac.uk/id/eprint/48489
DOI:

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