The G0/G1 switch gene 2 is a novel PPAR target gene

Zandbergen, Fokko, Mandard, Stéphane, Escher, Pascal, Tan, Nguan Soon, Patsouris, David, Jatkoe, Tim, Rojas-Caro, Sandra, Madore, Steve, Wahli, Walter, Tafuri, Sherrie, Müller, Michael ORCID: and Kersten, Sander (2005) The G0/G1 switch gene 2 is a novel PPAR target gene. Biochemical Journal, 392 (Pt 2). pp. 313-24. ISSN 0264-6021

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PPARs (peroxisome-proliferator-activated receptors) alpha, beta/delta and gamma are a group of transcription factors that are involved in numerous processes, including lipid metabolism and adipogenesis. By comparing liver mRNAs of wild-type and PPARalpha-null mice using microarrays, a novel putative target gene of PPARalpha, G0S2 (G0/G1 switch gene 2), was identified. Hepatic expression of G0S2 was up-regulated by fasting and by the PPARalpha agonist Wy14643 in a PPARalpha-dependent manner. Surprisingly, the G0S2 mRNA level was highest in brown and white adipose tissue and was greatly up-regulated during mouse 3T3-L1 and human SGBS (Simpson-Golabi-Behmel syndrome) adipogenesis. Transactivation, gel shift and chromatin immunoprecipitation assays indicated that G0S2 is a direct PPARgamma and probable PPARalpha target gene with a functional PPRE (PPAR-responsive element) in its promoter. Up-regulation of G0S2 mRNA seemed to be specific for adipogenesis, and was not observed during osteogenesis or myogenesis. In 3T3-L1 fibroblasts, expression of G0S2 was associated with growth arrest, which is required for 3T3-L1 adipogenesis. Together, these data indicate that G0S2 is a novel target gene of PPARs that may be involved in adipocyte differentiation.

Item Type: Article
Uncontrolled Keywords: adipocytes,adipogenesis,animals,base sequence,cell cycle proteins,cell line,endoplasmic reticulum,gene deletion,hepatocytes,humans,liver,male,mice,molecular sequence data,oligonucleotide array sequence analysis,ppar alpha,promoter regions, genetic,protein transport,rna, messenger,rats,response elements,sequence homology, nucleic acid,substrate specificity,up-regulation
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 07 Jul 2014 12:04
Last Modified: 24 Oct 2022 06:08
DOI: 10.1042/BJ20050636

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