Hepatocyte caspase-8 is an essential modulator of steatohepatitis in rodents

Hatting, Maximilian, Zhao, Gang, Schumacher, Fabienne, Sellge, Gernot, Al Masaoudi, Malika, Gaβler, Nikolaus, Boekschoten, Mark, Müller, Michael, Liedtke, Christian, Cubero, Francisco Javier and Trautwein, Christian (2013) Hepatocyte caspase-8 is an essential modulator of steatohepatitis in rodents. Hepatology, 57 (6). pp. 2189-2201. ISSN 1527-3350

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Abstract

In human and murine models of nonalcoholic steatohepatitis (NASH), increased hepatocyte apoptosis is a critical mechanism contributing to inflammation and fibrogenesis. Caspase 8 (Casp8) is essential for death-receptor-mediated apoptosis activity and therefore its modulation might be critical for the pathogenesis of NASH. The aim was to dissect the role of hepatocyte Casp8 in a murine model of steatohepatitis. We generated hepatocyte-specific Casp8 knockout (Casp8(Δhep) ) mice. Animals were fed with a methionine-choline-deficient (MCD) diet. Liver injury was assessed by histopathological analysis, apoptotic death, serum alanine aminotransferase (ALT), fluorescent-activated cell sorter (FACS), analysis of liver infiltration and inflammation, reactive oxygen species (ROS), and liver fibrosis. MCD feeding triggered steatosis, hepatic lipid storage, and accumulation of free fatty acid (FFA) in wildtype (WT) livers, which were significantly reduced in Casp8(Δhep) animals. Additionally, lack of Casp8 expression in hepatocytes reduced the MCD-dependent increase in apoptosis and decreased expression of proinflammatory cytokines as well as hepatic infiltration. As a consequence, ROS production was lower, leading to a reduction in the progression of liver fibrosis in Casp8(Δhep) livers. Conclusion: Selective ablation of Casp8 in hepatocytes ameliorates development of NASH by modulating liver injury. Casp8-directed therapy might be a plausible treatment for patients with steatohepatitis. (HEPATOLOGY 2013;57:2189-2201).

Item Type: Article
Additional Information: Copyright © 2013 American Association for the Study of Liver Diseases.
Uncontrolled Keywords: animals,apoptosis,caspase 8,fatty liver,fibrosis,hepatocytes,lipid metabolism,liver,male,mice,mice, knockout,oxidative stress
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 10 Jun 2014 20:30
Last Modified: 14 Sep 2020 07:03
URI: https://ueaeprints.uea.ac.uk/id/eprint/47643
DOI: 10.1002/hep.26271

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