HFE H63D, C282Y and AGTR1 A1166C polymorphisms and brain white matter lesions in the aging brain

Gebril, Ola H, Kirby, Janine, Savva, George, Brayne, Carol and Ince, Paul G (2011) HFE H63D, C282Y and AGTR1 A1166C polymorphisms and brain white matter lesions in the aging brain. Journal of Neurogenetics, 25 (1-2). pp. 7-14. ISSN 1563-5260

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Abstract

Incidental white matter lesions (WML) are a common neuroradiological finding in elderly people and have been linked to dementia and depression. Various mechanisms including hypoxia and increased production of reactive oxygen species (ROS) are implicated in the etiology of WML. The hemochromatosis (HFE) gene p.H63D and p.C282Y polymorphisms have been linked to dysregulation of iron metabolism and increased levels of ROS, whereas Angiotensin II receptor 1 (AGTR1) c.1166A → C polymorphism is known as a vascular risk factor. These genetic polymorphisms were characterized in brains donated to the UK MRC Cognitive Function and Ageing Study (CFAS) to assess their potential role in the risk for development of age-related WML. The study cohort comprised 258 brain donated to CFAS. WML severity was assessed in the postmortem brain donations using magnetic resonance imaging (MRI) scans and scored using the Scheltens' scale. Polymerase chain reaction (PCR) amplification of extracted DNA followed by restriction enzyme digestion was used to genotype the samples. Genotypes were validated using direct sequencing in a smaller sample. The results show that HFE p.H63D polymorphism is not associated with WML severity in the whole cohort. However, there is a significant association of the D allele with severity of WML in noncarriers of the APOE ε4 allele. No association is demonstrated between the HFE p.C282Y nor the AGTR1 c.1166A → C polymorphisms and WML severity. The HFE gene appears to be a genetic risk factor for severe aging WML independently of the APOE ε4 genotype. This would support the role of iron-related oxidative stress, in addition to previously studied factors, e.g., hypoxia as potential risk factors for developing prominent aging WML.

Item Type: Article
Uncontrolled Keywords: aged,aged, 80 and over,aging,amino acids,brain,female,gene frequency,genetic predisposition to disease,genotype,histocompatibility antigens class i,humans,leukoencephalopathies,magnetic resonance imaging,male,membrane proteins,middle aged,polymorphism, single nucleotide,receptor, angiotensin, type 1,risk factors
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Depositing User: Pure Connector
Date Deposited: 06 Jan 2014 14:32
Last Modified: 21 Apr 2020 22:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/47062
DOI: 10.3109/01677063.2011.556206

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