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de Wit, Nicole J. W., Boekschoten, Mark V., Bachmair, Eva-Maria, Hooiveld, Guido J. E. J., de Groot, Philip J., Rubio-Aliaga, Isabel, Daniel, Hannelore and Müller, Michael 
ORCID: https://orcid.org/0000-0002-5930-9905
(2011)
Dose-dependent effects of dietary fat on development of obesity in relation to intestinal differential gene expression in C57BL/6J mice.
PLoS One, 6 (4).
ISSN 1932-6203
Abstract
Excessive intake of dietary fat is known to be a contributing factor in the development of obesity. In this study, we determined the dose-dependent effects of dietary fat on the development of this metabolic condition with a focus on changes in gene expression in the small intestine. C57BL/6J mice were fed diets with either 10, 20, 30 or 45 energy% (E%) derived from fat for four weeks (n = 10 mice/diet). We found a significant higher weight gain in mice fed the 30E% and 45E% fat diet compared to mice on the control diet. These data indicate that the main shift towards an obese phenotype lies between a 20E% and 30E% dietary fat intake. Analysis of differential gene expression in the small intestine showed a fat-dose dependent gradient in differentially expressed genes, with the highest numbers in mice fed the 45E% fat diet. The main shift in fat-induced differential gene expression was found between the 30E% and 45E% fat diet. Furthermore, approximately 70% of the differentially expressed genes were changed in a fat-dose dependent manner. Many of these genes were involved in lipid metabolism-related processes and were already differentially expressed on a 30E% fat diet. Taken together, we conclude that up to 20E% of dietary fat, the small intestine has an effective 'buffer capacity' for fat handling. From 30E% of dietary fat, a switch towards an obese phenotype is triggered. We further speculate that especially fat-dose dependently changed lipid metabolism-related genes are involved in development of obesity.
| Item Type: | Article |
|---|---|
| Additional Information: | © 2011 de Wit et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Uncontrolled Keywords: | animals,dietary fats,eating,gene expression,small intestine,male,mice,inbred c57bl mice,obesity,weight gain,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 18 Dec 2013 12:00 |
| Last Modified: | 24 Oct 2022 05:54 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/47051 |
| DOI: | 10.1371/journal.pone.0019145 |
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