A sporulation specific, sigF -dependent protein, SspA, affects septum positioning in Streptomyces coelicolor

Tzanis, Angelos, Dalton, Kate A., Hesketh, Andrew, Den Hengst, Chris D., Buttner, Mark J., Thibessard, Annabelle and Kelemen, Gabriella H. (2014) A sporulation specific, sigF -dependent protein, SspA, affects septum positioning in Streptomyces coelicolor. Molecular Microbiology, 91 (2). pp. 363-380. ISSN 0950-382X

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Abstract

The RNA polymerase sigma factor SigF controls late development during sporulation in the filamentous bacterium Streptomyces coelicolor. The only known SigF-dependent gene identified so far, SCO5321, is found in the biosynthetic cluster encoding spore pigment synthesis. Here we identify the first direct target for SigF, the gene sspA, encoding a sporulation-specific protein. Bioinformatic analysis suggests that SspA is a secreted lipoprotein with two PepSY signature domains. The sspA deletion mutant exhibits irregular sporulation septation and altered spore shape, suggesting that SspA plays a role in septum formation and spore maturation. The fluorescent translational fusion protein SspA–mCherry localized first to septum sites, then subsequently around the surface of the spores. Both SspA protein and sspA transcription are absent from the sigF null mutant. Moreover, in vitro transcription assay confirmed that RNA polymerase holoenzyme containing SigF is sufficient for initiation of transcription from a single sspA promoter. In addition, in vivo and in vitro experiments showed that sspA is a direct target of BldD, which functions to repress sporulation genes, including whiG, ftsZ and ssgB, during vegetative growth, co-ordinating their expression during sporulation septation.

Item Type: Article
Additional Information: © 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funded by BBSRC. Grant Number: BBS/B/06938
Uncontrolled Keywords: sporulation,septum positioning,sigf,bldd,streptomyces development
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 18 Dec 2013 10:28
Last Modified: 21 Apr 2020 22:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/47002
DOI: 10.1111/mmi.12466

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