Bitter melon extract inhibits proliferation of Trypanosoma brucei bloodstream forms in vitro

Phillips, Elizabeth A, Sexton, Darren W and Steverding, Dietmar (2013) Bitter melon extract inhibits proliferation of Trypanosoma brucei bloodstream forms in vitro. Experimental Parasitology, 133 (3). pp. 353-6. ISSN 1090-2449

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Abstract

Trypanosoma brucei is the causative agent of sleeping sickness, a fatal disease prevalent in sub-Saharan Africa. The few currently available drug treatments are dated and face problems with toxicity and resistance. For these reasons, there is an urgent need for the development of new chemotherapies for the treatment of sleeping sickness. In this study, we investigated the trypanocidal activity of bitter melon extract. Recently, it has been shown that bitter melon extracts display cytotoxic activity towards different cancer cell lines. However, agents exhibiting anti-tumour activity are usually also inhibiting the growth of T. brucei. Treatment of bloodstream forms of T. brucei with extracts prepared from Chinese and Indian bitter melon varieties resulted in a decrease in cell proliferation. In contrast, human myeloid leukaemia HL-60 cells were 3-6 times less sensitive to the extracts than trypanosomes. Initial fractionation of bitter melon extracts indicated that the trypanocidal activity of the extract is associated with at least two different classes of substances: one class of larger molecular weight compounds (>3 kDa) causing rapid lysis of trypanosomes and one class of smaller molecular weight compounds (

Item Type: Article
Additional Information: Copyright © 2013 Elsevier Inc. All rights reserved.
Uncontrolled Keywords: cell cycle,dose-response relationship, drug,flow cytometry,hl-60 cells,humans,momordica charantia,parasitic sensitivity tests,plant extracts,trypanocidal agents,trypanosoma brucei brucei
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 06 Jan 2014 14:38
Last Modified: 21 Apr 2020 22:40
URI: https://ueaeprints.uea.ac.uk/id/eprint/46828
DOI: 10.1016/j.exppara.2012.12.004

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