Evidence for an enhancing effect of alginate on iron availability in Caco-2 cells

Wawer, Anna A, Sharp, Paul A, Perez-Moral, Natalia and Fairweather-Tait, Susan J (2012) Evidence for an enhancing effect of alginate on iron availability in Caco-2 cells. Journal of Agricultural and Food Chemistry, 60 (45). pp. 11318-22. ISSN 0021-8561

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Abstract

The potential use of alginates as a vehicle for water-soluble (bioavailable) iron for fortifying food products was examined using a Caco-2 cell model system. Cell monolayers were exposed to alginates with various mannuronic to guluronic acid ratios at three different concentrations, and cellular ferritin was measured as a surrogate marker of iron uptake into the cell. Ferritin concentrations were significantly higher when the cells were treated with ferric ammonium citrate and 0.5 and 1% w/v (but not 0.1%) alginate, but were unaffected by mannuronic/guluronic acid ratios. The enhancing effect of ascorbic acid was maintained with 0.1% alginate and significantly increased with 0.5 and 1% alginate, whereas the inhibitory effect of tannic acid was significantly reduced with 0.5% alginate. Alginate beads delivered available iron to Caco-2 cells, indicating that they are a promising vehicle for soluble iron with potential use in food fortification programs.

Item Type: Article
Uncontrolled Keywords: alginates,biological availability,biological transport,caco-2 cells,ferritins,humans,iron
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 20 Jan 2014 16:12
Last Modified: 21 Apr 2020 22:33
URI: https://ueaeprints.uea.ac.uk/id/eprint/46227
DOI: 10.1021/jf3031309

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