The SseC translocon component in Salmonella enterica serovar Typhimurium is chaperoned by SscA

Cooper, Colin A, Mulder, David T, Allison, Sarah E, Pilar, Ana Victoria C and Coombes, Brian K (2013) The SseC translocon component in Salmonella enterica serovar Typhimurium is chaperoned by SscA. BMC Microbiology, 13.

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Abstract

Background: Salmonella enterica is a causative agent of foodborne gastroenteritis and the systemic disease known as typhoid fever. This bacterium uses two type three secretion systems (T3SSs) to translocate protein effectors into host cells to manipulate cellular function. Salmonella pathogenicity island (SPI)-2 encodes a T3SS required for intracellular survival of the pathogen. Genes in SPI-2 include apparatus components, secreted effectors and chaperones that bind to secreted cargo to coordinate their release from the bacterial cell. Although the effector repertoire secreted by the SPI-2 T3SS is large, only three virulence-associated chaperones have been characterized. Results: Here we report that SscA is the chaperone for the SseC translocon component. We show that SscA and SseC interact in bacterial cells and that deletion of sscA results in a loss of SseC secretion, which compromises intracellular replication and leads to a loss of competitive fitness in mice. Conclusions: This work completes the characterization of the chaperone complement within SPI-2 and identifies SscA as the chaperone for the SseC translocon.

Item Type: Article
Additional Information: © 2013 Cooper et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: salmonella,pathogenesis,chaperone,translocon,t3ss
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 20 Jan 2014 15:58
Last Modified: 31 Jul 2020 23:36
URI: https://ueaeprints.uea.ac.uk/id/eprint/46114
DOI: 10.1186/1471-2180-13-221

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