Defining the bacteroides ribosomal binding site

Wegmann, Udo, Horn, Nikki and Carding, Simon R (2013) Defining the bacteroides ribosomal binding site. Applied and Environmental Microbiology, 79 (6). pp. 1980-9. ISSN 0099-2240

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The human gastrointestinal tract, in particular the colon, hosts a vast number of commensal microorganisms. Representatives of the genus Bacteroides are among the most abundant bacterial species in the human colon. Bacteroidetes diverged from the common line of eubacterial descent before other eubacterial groups. As a result, they employ unique transcription initiation signals and, because of this uniqueness, they require specific genetic tools. Although some tools exist, they are not optimal for studying the roles and functions of these bacteria in the human gastrointestinal tract. Focusing on translation initiation signals in Bacteroides, we created a series of expression vectors allowing for different levels of protein expression in this genus, and we describe the use of pepI from Lactobacillus delbrueckii subsp. lactis as a novel reporter gene for Bacteroides. Furthermore, we report the identification of the 3' end of the 16S rRNA of Bacteroides ovatus and analyze in detail its ribosomal binding site, thus defining a core region necessary for efficient translation, which we have incorporated into the design of our expression vectors. Based on the sequence logo information from the 5' untranslated region of other Bacteroidales ribosomal protein genes, we conclude that our findings are relevant to all members of this order.

Item Type: Article
Uncontrolled Keywords: 5' untranslated regions,bacteroides,binding sites,gene expression,genetic vectors,genetics, microbial,molecular biology,protein biosynthesis,rna, messenger,rna, ribosomal,ribosomes
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 16 Dec 2013 15:14
Last Modified: 24 Oct 2022 05:29
DOI: 10.1128/AEM.03086-12

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