The purMN genes of Rhizobium leguminosarum and a superficial link with siderophore production

Stevens, James B., de Luca, Nicola G., Beringer, John E., Ringer, John P., Yeoman, Kay H. and Johnston, Andrew W. B. (2000) The purMN genes of Rhizobium leguminosarum and a superficial link with siderophore production. Molecular Plant-Microbe Interactions, 13 (2). pp. 228-231. ISSN 0894-0282

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Abstract

We isolated a mutant of R. leguminosarum initially on the basis of reduced production of the siderophore Vicibactin on chrome azurol sulfonate (CAS)/agar indicator plates. The mutation was in the purMN operon and the mutant was shown to be an adenine auxotroph and defective for nodulation of peas. The siderophore defect appears to be trivial, being due to diminished growth of the auxotroph on agar-based minimal medium, which contains unknown contaminant(s) that allow it grow poorly. Transcriptional fusions showed that purMN was transcribed at relatively high levels in media containing purines. Expression was enhanced, approximately twofold, if purines were omitted.

Item Type: Article
Uncontrolled Keywords: n 2 fixation,purine auxotrophy
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 26 Nov 2013 13:00
Last Modified: 21 Apr 2020 22:13
URI: https://ueaeprints.uea.ac.uk/id/eprint/44546
DOI: 10.1094/MPMI.2000.13.2.228

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