Is the fur gene of Rhizobium leguminosarum essential?

de Luca, Nicola G., Wexler, Margaret, Pereira, Maria J., Yeoman, Kay H. and Johnston, Andrew W. B. (1998) Is the fur gene of Rhizobium leguminosarum essential? FEMS Microbiology Letters, 168 (2). pp. 289-295. ISSN 0378-1097

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Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of Fe in the medium, but in one Rhizobium strain it was transcribed at a low level. Attempts to isolate a fur knockout mutant failed, suggesting that the gene is essential for free-living growth. In other bacteria, certain fur mutations confer manganese resistance; however, none of the manganese-resistant mutants of R. leguminosarum which we isolated was corrected by the cloned fur gene. When the cloned R. leguminosarum fur gene was introduced into a fur mutant of Escherichia coli, it caused some Fe-dependent reduction in the amount of siderophore, indicating that it can function heterologously. Copyright (C) 1998 Federation of European Microbiological Societies.

Item Type: Article
Uncontrolled Keywords: fur,gene regulation,iron,manganese resistance,nitrogen fixation,rhizobium
Faculty \ School:
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Social Sciences > Research Groups > Research in Mathematics Education
Depositing User: Pure Connector
Date Deposited: 26 Nov 2013 12:32
Last Modified: 24 Oct 2022 05:05

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